General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter U2OS β-Catenin Nuclear Translocation Cell Line, 2 vials. (93-0743C3)
The PathHunter U2OS β-Catenin Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the Wnt-FzGSK3β pathway. This cell line enables assessment of ligand (e.g. wnt 3a) based pathway activation via detection of resulting β-Catenin nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged β-Catenin and nuclear localized EA. Upon pathway activation, induced nuclear translocation of β-Catenin leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Eurofins Discoverx KILR SKOV3 Cell Pool, 2 vials. (97-1007P023)
The KILR SKOV3 cell pool is a stable, engineered target cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell pool is a heterogeneous population of SKOV3 cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.
Genscript Corporation CHO-K1/ADRA2B/Gqi5 Stable Cell
Adrenoceptors are 7-transmembrane receptors which mediate the central and peripheral actions of the neurotransmitter noradrenaline (norepinephrine) and the hormone and neurotransmitter adrenaline (epinephrine) Based on both pharmacological and molecular evidence they are divided into three major types - 1 2 and The 2 adrenoceptors include 3 subtypes - 2A 2B and 2C - each of which couples to G sub i/o /sub protein primarily br The 2B adrenoceptors (ADRA2B) is expressed in the kidney liver brain lung heart and skeletal muscle The development of 2B and 2C knock-out mice has shown that these two subtypes are not involved in the central hypotensive response to 2 agonists
Eurofins Discoverx PathHunter CHO-K1 GPR12 β-Arrestin Orphan GPCR Cell Line, 2 vials. (93-0329C2A)
The PathHunter GPR12 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of GPR12 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR12 and EA-tagged β-Arrestin-2. Activation of GPR12 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. This is an orphan receptor without a known ligand. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Miltenyi Biotec, Inc. Exosome Isolation Kit CD63, mouse
Kit for manual isolation of exosomes or extracellular vesicles
Eurofins Discoverx KILR SK-MEL-28 Cell Pool, 2 vials. (97-1017P031)
The KILR SK-MEL-28 cell pool is a stable, engineered target cell pool, which closely reflects the heterogeneous native protein expression of tumor environments, used to measure direct cell death mediated by immunooncology treatments (e.g. checkpoint modulators, bispecifics, CAR-T, etc.) via Antibody Dependent Cell-mediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), and Antibody Dependent Cell Phagocytosis (ADCP). This product uses EFC technology. The cell pool is a heterogeneous population of SK-MEL-28 cells that overexpress a ProLabel-tagged housekeeping gene. Upon effector cells-mediated cell lysis, ProLabel is released to the media, where EA in the detection kit produces an EFC signal proportional to the level of ProLabel released. This allows for calculation of percent lysis of the KILR cell pool. This stable cell pool is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kilr.
Charles River Labs Cell Solutions PB CD3 CELLS- POS SEL CRYO 10M
For Research Use Only. Human Normal Peripheral Blood CD3+ Pan T Cells - Cryopreserved, 10 million cells/vial. Isolated using POSITIVE immunomagnetic cell separation procedures.
Eurofins Discoverx FFAR1 (GPR40) Gq Calcium Signaling Cell-Based Assay Kit (CHO-K1)
The FFAR1 (GPR40) Gq Stable Cell Line Calcium Signaling Assay (CHO-K1) contains a stable clonal cell line used to measure FFAR1 (GPR40) (GPCR) activation via an increase in intracellular calcium. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter CHO-K1 mGHSR1a β-Arrestin Cell Line, 2 vials. (93-0605C2)
The PathHunter mGHSR1a cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. grehlin) based activation of mGHSR1a GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGHSR1a and EA-tagged β-Arrestin-2. Activation of mGHSR1a stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 GLP2R β-Arrestin Cell Line, 2 vials. (93-0572C2)
The PathHunter GLP2R cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Creative Biolabs Human OVCAR-8 Tumor Cell Line, Tumor Cell Line, OVCAR-8, Ovarian Adenocarcinoma, Epithelial, Adherent morphology, Storage: Liquid nitrogen (-150°C to -190°C), Applications: Cancer research, Drug screening
Human OVCAR-8 Tumor Cell Line, Tumor Cell Line, OVCAR-8, Ovarian Adenocarcinoma, Epithelial, Adherent morphology, Storage: Liquid nitrogen (-150°C to -190°C), Applications: Cancer research, Drug screening
Eurofins Discoverx cAMP Hunter CHO-K1 GLP1R Gs Cell Line, 2 vials. (95-0062C2)
The cAMP Hunter GLP1R Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP1R, a naturally Gs coupled GPCR. Upon activation of GLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 FPRL1 Gi Cell Line, 2 vials. (95-0044C2)
The cAMP Hunter FPRL1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand based activation of FPRL1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FPRL1, a naturally Gi/Go coupled GPCR. Upon activation of FPRL1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter AGTRL1 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0710C3)
The PathHunter AGTRL1 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of AGTRL1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AGTRL1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AGTRL1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.